Acetonitrile + 0.1% formic acid, Optima LC/MS Grade

An acetonitrile solution with 0.1% Optima LC/MS Grade formic acid is a ready-made mixture for use in liquid chromatography and mass spectrometry. The reagent is in demand in proteomics for the separation of peptides, in pharmaceutical quality control, as well as in the analysis of pesticides and other trace pollutants. The key advantage of the finished mixture is the exclusion of the stage of manual acid addition. The user receives a solvent with a guaranteed accurate concentration of formic acid (0.095–0.105% by volume), which eliminates dosing errors and reduces variability between batches. This approach is especially important for validated techniques where strict reproducibility of the mobile phase composition is required. Formic acid plays the role of an ion-paired reagent in LC-MS analysis. It improves the ionization of analytes in the positive ionization mode, contributing to the formation of protonated [M+H]⁺ molecules. The additive suppresses the interaction of the main compounds with the free silanol groups of the stationary phase, which leads to a more symmetrical peak shape and increased separation efficiency. The product undergoes strict quality control. The solvent is filtered through a 0.2 micron membrane to remove mechanical particles. The content of metal impurities is controlled at the level of billionths, which prevents the formation of undesirable metal adducts in the mass spectra. Organic pollutants are tested in positive and negative ionization modes to confirm the suitability of the solvent for the most sensitive MS applications.