Trifluoroacetic Acid (TFA), Optima LC/MS Grade

Optima LC/MS Grade trifluoroacetic acid is a strong volatile organic acid widely used as a mobile phase modifier in high performance liquid chromatography and liquid chromato-mass spectrometry. The reagent is indispensable for the separation of peptides, proteins and other biomolecules in the reversed phase mode. The key feature of TFA is its ability to function as an ion-paired reagent. The addition of acid suppresses the interaction of positively charged amino groups of peptides with free silanol groups of the stationary phase. This leads to a significant improvement in the shape of chromatographic peaks and an increase in resolution when separating complex peptide mixtures. The product demonstrates excellent spectrophotometric characteristics required to work with UV detectors. The absorption at a wavelength of 210 nanometers does not exceed 0.55 units, at 254 nanometers — 0.003 units. High UV transmission allows you to work at low wavelengths, where peptide bonds give the maximum signal, without loss of sensitivity. The content of metal impurities is carefully controlled at the level of billionths. This approach eliminates the formation of undesirable metal adducts in the mass spectra and guarantees the purity of the ion source during long-term analytical series. The residue after evaporation is no more than 1 ppm, which confirms the high purity of the product. The purity of the reagent exceeds 99.5 percent. The product is packaged in glass ampoules under an inert atmosphere, which prevents hydrolysis and contamination. Trifluoroacetic acid is a corrosive substance and requires strict compliance with safety measures.